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r2018a  (MathWorks Inc)
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statistics toolbox function non-negative matrix factorization (nnmf)  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
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statistical toolbox r2010b  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
Statistical Toolbox R2010b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistics and fuzzy logic tool packages  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
Statistics And Fuzzy Logic Tool Packages, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kstest2 function  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
Kstest2 Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c matlab statistics  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
C Matlab Statistics, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r2019a  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
R2019a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistics toolbox release 2012  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
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matlab 9.9.0.c  (MathWorks Inc)
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Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy <t>(NNMF</t> Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).
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Image Search Results


Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy (NNMF Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).

Journal: Biomaterials and Biosystems

Article Title: In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy

doi: 10.1016/j.bbiosy.2022.100059

Figure Lengend Snippet: Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy (NNMF Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).

Article Snippet: Following pre-processing, spectral models were developed using the MATLAB statistics toolbox function non-negative matrix factorization (NNMF) ( c) with in vivo spectra ( a), ex vivo , and reference spectra ( b) as input.

Techniques: Immunohistochemical staining, Raman Spectroscopy, Derivative Assay, Ex Vivo, In Vivo